Inhibitory effect of cobalt oxide nanoparticles with biosynthetic licorice against bacterial species isolated from urinary tract infection

Abstract

Conclusion 120 management samples were collected for samples from patients of Samarra Hospital and some clinics in Salah Al-Din Governorate / Samarra for the period from January 2023 until the end of June 2024. The swabs were planted on the middle of the MacConkey and the blood agar, then incubated for 24 hours at room temperature, and the isolates were diagnosed based on a set of phenotypic characteristics of the developing isolates and biochemical tests, distributed over 20 samples, i.e. 20% of urinary tract infection isolates in which no bacterial growth occurred (Nigative UTI), while the 80%, i.e. 80%, occurred in which bacterial growth, The nanoparticles were prepared in two ways, the first chemical synthetic method and the second green method using the licorice plant glabra glycyrrhiza The results of the diagnostic tests were that the bacteria coli Escherichia, which are 35 isolates, i.e. (43.75)%, while the bacteria Klebsiella spp appeared by 15 samples, i.e. (18.75) %, and Staphylococcus aureus bacteria appeared by 15 samples and by (18.75) %. As for the bacteria Staphylococcus.epidermidis, 10 samples appeared by (12.5)%, while the yeast Candida albicans appeared 5 samples by (6.5)% The bacterial species that have been isolated are among the most common types that cause urinary tract infection (UTI), as the bacteria that are the most present of the species that have been isolated are the bacteria coli Escherichia, followed by Klebsiella spp bacteria, Staphylococcus aureus, Staphylococcus.epidermidis and Candida albicans yeast respectively, and it was found that the plant extract with the nanomaterial cobalt oxide With licorice glabra glycyrrhiza prepared by the second method, Green synthesis nanoparticles by plant extract, is more efficient than the nanomaterial prepared by the first method, Artificial roads nanoparticle in inhibiting bacterial isolates, the proportion of E. coli bacteria was (32) mm, while S. aureus bacteria were (28) mm. And bacteria Klebsiella 31)) Extract nano-oxide cobalt with licorice, by the second green method T2)) was one of the best types of work and the effect of its inhibition is high on the bacterial species used, where it was observed to contain plant extracts The following phytochemical analysis ingredients: alkaloids, glycosides, phenols, saponins, flavonoids and tanginations. Successful use of plant extract Nanocomposites cobalt and glabra glycyrrhiza in the treatment of infections caused by urinary tract infection caused by E. coli bacteria with cobalt oxide extract with licorice. Then tests were carried out for cobalt oxide nanosolutions prepared by the second method, tests were AFM, UV, FTIR, the nanomaterials were of a smaller size of 5–12nm called quantum dot, and no agglomeration occurred, it was found that the MTT, cytotoxicity assay for Nanocomposites cobalt solutions used to inhibit bacterial species showed that the substance is non-toxic to the solution for the treatment of infections caused by Escherichia coli bacteria and Staphylococcus aureus.